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Antibodies

Agrn Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC11305-50UG
100 ug
£454.00
CAC11305-100UG
About this Product
SKU:
CAC11305
Additional Names:
AGRIN|Agrin C-terminal 22 kDa fragment|Agrin proteoglycan|AGRIN_HUMAN|Agrn|C22|C90|FLJ45064|OTTHUMP00000044043
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Agrn Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
AGRN
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Immunofluorescence staining of Hela cells with CAC11305 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot; ,Positive WB detected in: A549 whole cell lysate, U87 whole cell lysate, 293 whole cell lysate; ,All lanes: AGRN antibody at 7.5ug/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 218, 206, 217, 215 kDa; ,Observed band size: 218 kDa
IHC image diluted at 1:400 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.