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Antibodies

Acaa2 Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC11123-50UG
100 ug
£454.00
CAC11123-100UG
About this Product
SKU:
CAC11123
Additional Names:
3 ketoacyl CoA thiolase|3 ketoacyl CoA thiolase mitochondrial|3-ketoacyl-CoA thiolase|3-oxoacyl-CoA thiolase|ACAA 2|Acaa2|Acetyl CoA acyltransferase|Acetyl CoA acyltransferase 2|Acetyl Coenzyme A acyltransferase 2|Acetyl-CoA acyltransferase|Beta ketothiolase|Beta-ketothiolase|DSAEC|mitochondrial|Mitochondrial 3 oxoacyl CoA thiolase|Mitochondrial 3 oxoacyl Coenzyme A thiolase|Mitochondrial 3-oxoacyl-CoA thiolase|T1|THIM_HUMAN
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Acaa2 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF, IP.
Host:
Rabbit
Immunogen:
ACAA2
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Mouse, Rat
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue; All lanes: ACAA2 antibody at 2.4ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 42 kDa; Observed band size: 42 kDa
IHC image diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of HepG2 cells using CAC11123 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating ACAA2 in A549 whole cell lysate ; , Lane 1: Rabbit control IgG (1ug) instead in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11123 (6ug) + A549 whole cell lysate (500ug); , Lane 3: A549 whole cell lysate (10ug)