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Antibodies

Atg4D Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC10480-50UG
100 ug
£454.00
CAC10480-100UG
About this Product
SKU:
CAC10480
Additional Names:
9830134P12Rik|APG4|APG4 autophagy 4 homolog D|APG4 D|APG4D|ATG4 autophagy related 4 homolog D (S. cerevisiae)|Atg4d|ATG4D_HUMAN|Atg4dl|AUT like 4 cysteine endopeptidase|AUT-like 4 cysteine endopeptidase|AUTL4|Autophagin 4|Autophagin-4|autophagy 4|autophagy related 4D|Autophagy related cysteine endopeptidase 4|Autophagy related protein 4 homolog D|Autophagy-related cysteine endopeptidase 4|Autophagy-related protein 4 homolog D|cysteine peptidase|Cysteine protease ATG4D|Cysteine protease involved in autophagy|D|homolog of|MGC31226|S. cerevisiae
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Atg4D Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
ATG4D
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, Mouse heart tissue; All lanes: ATG4D antibody at 2.8ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 53, 17 kDa; Observed band size: 53 kDa
IHC image diluted at 1:700 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:700 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of PC-3 cells with CAC10480 at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).