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Antibodies

Cd3E Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC09127-50UG
100 ug
£454.00
CAC09127-100UG
About this Product
SKU:
CAC09127
Additional Names:
CD3 epsilon|CD3e|CD3e antigen|CD3E antigen epsilon polypeptide|CD3e antigen epsilon polypeptide (TiT3 complex)|CD3e molecule|CD3e molecule epsilon|CD3E_HUMAN|epsilon (CD3 TCR complex)|epsilon (CD3-TCR complex)|epsilon subunit|IMD18|T cell antigen receptor complex epsilon subunit of T3|T cell surface antigen T3/Leu 4 epsilon chain|T cell surface glycoprotein CD3 epsilon chain|T-cell surface antigen T3/Leu-4 epsilon chain|T-cell surface glycoprotein CD3 epsilon chain|T3E|TCRE
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Cd3E Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
CD3E
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
IHC image of CAC09127 diluted at 1:80 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:80 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of PC-3 cells with CAC09127 at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; , All lanes: CD3E antibody at 1.5ug/ml + Jurkat whole cell lysate; , Secondary; , Goat polyclonal to rabbit IgG at 1/10000 dilution; , Predicted band size: 24 kDa; , Observed band size: 24 kDa