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Antibodies

Dnaja1 Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC08366-50UG
100 ug
£454.00
CAC08366-100UG
About this Product
SKU:
CAC08366
Additional Names:
DJ 2|DJ2|DjA1|DnaJ (Hsp40) homolog|DnaJ homolog subfamily A member 1|DnaJ protein homolog 2|DNAJ2|Dnaja1|DNJA1_HUMAN|hDJ 2|HDJ-2|HDJ2|Heat shock 40 kDa protein 4|heat shock protein DNAJ like 2|Heat shock protein J2|HSDJ|HSJ-2|HSJ2|HSPF4|Human DnaJ protein 2|member 1|NEDD7|Neural precursor cell expressed developmentally down regulated 7|OTTHUMP00000021193|subfamily A
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Dnaja1 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
DNAJA1
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: Hela whole cell lysate; All lanes: DNAJA1 antibody at 3.4ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 45, 38 kDa; Observed band size: 45, 38 kDa
IHC image diluted at 1:1000 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC08366 at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).