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Antibodies

Cflar Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC08309-50UG
100 ug
£454.00
CAC08309-100UG
About this Product
SKU:
CAC08309
Additional Names:
c FLIP|c FLIPL|c FLIPR|c FLIPS|c-FLIP|c-FLIPL|c-FLIPR|c-FLIPS|CASH|CASP8 and FADD like apoptosis regulator|CASP8 and FADD-like apoptosis regulator subunit p12|CASP8AP1|Caspase homolog|Caspase like apoptosis regulatory protein|Caspase related inducer of apoptosis|Caspase-eight-related protein|Caspase-like apoptosis regulatory protein|Casper|cellular flice inhibitory protein|Cellular FLICE-like inhibitory protein|CFLA|Cflar|CFLAR_HUMAN|CLARP|FADD like anti apoptotic molecule|FADD-like antiapoptotic molecule 1|FLAME|FLAME-1|FLAME1|FLIP|I FLICE|I-FLICE|Inhibitor of FLICE|MACH-related inducer of toxicity|MRIT|OTTHUMP00000163715|OTTHUMP00000206475|OTTHUMP00000206476|OTTHUMP00000206478|OTTHUMP00000206479|OTTHUMP00000206480|OTTHUMP00000206482|OTTHUMP00000207360|Usurpin|Usurpin beta
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Cflar Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
CFLAR
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Immunofluorescence staining of Hela cells with CAC08309 at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot; ,Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, 293T whole cell lysate; ,All lanes: CFLAR antibody at 4ug/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 56, 26, 42, 28, 52, 51, 40, 31, 35, 53, 34, 24, 50, 45 kDa; ,Observed band size: 56 kDa
IHC image diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.