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Antibodies

Capn2 Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC08282-50UG
100 ug
£454.00
CAC08282-100UG
About this Product
SKU:
CAC08282
Additional Names:
Calcium activated neutral proteinase|Calcium activated neutral proteinase 2|Calcium-activated neutral protease 2|Calcium-activated neutral proteinase 2|CALP80|Calpain 2|Calpain 2 (m/II) large subunit|Calpain 2 catalytic subunit|Calpain 2 large catalytic subunit|Calpain 2 large subunit|Calpain large polypeptide L2|Calpain M type|Calpain M-type|Calpain-2 catalytic subunit|Calpain-2 large subunit|Calpain2|CAN2_HUMAN|CANP 2|CANP L2|CANP2|CANPL 2|CANPL2|CANPml|Capa2|CAPN 2|CAPN2|catalytic subunit|FLJ39928|large [catalytic] subunit|M calpain|M calpin|M type|M-calpain|mCANP|Millimolar calpain|Millimolar-calpain
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Capn2 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF, IP.
Host:
Rabbit
Immunogen:
CAPN2
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: 293T whole cell lysate; All lanes: CAPN2 antibody at 3.4ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 80, 72 kDa; Observed band size: 80 kDa
IHC image diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:300 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC08282 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).