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Antibodies

Cstb Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC08250-50UG
100 ug
£454.00
CAC08250-100UG
About this Product
SKU:
CAC08250
Additional Names:
CHROW21|CPI B|CPI-B|CST 6|CST6|CSTB|Cystatin B|Cystatin B (stefin B)|Cystatin-B|CYTB|CYTB_HUMAN|EPM1|EPM1A|Liver thiol proteinase inhibitor|PME|Stefin-B|STF B|STFB|ULD
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Cstb Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF, IP.
Host:
Rabbit
Immunogen:
CSTB
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Immunohistochemistry of paraffin-embedded human colon cancer using CAC08250 at dilution of 1:100
IHC image diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot; , Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, 293T whole cell lysate; , All lanes: CSTB antibody at 5.36ug/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 12 kDa; , Observed band size: 12 kDa
Immunofluorescence staining of HepG2 cells with CAC08250 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).