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Antibodies

Becn1 Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC07912-50UG
100 ug
£454.00
CAC07912-100UG
About this Product
SKU:
CAC07912
Additional Names:
APG6|ATG 6|ATG6|ATG6 autophagy related 6 homolog|Bcl-2-interacting protein beclin|Beclin 1 (coiled coil moesin like BCL2 interacting protein)|Beclin 1 autophagy related|Beclin-1|Beclin1|BECN 1|Becn1|BECN1_HUMAN|Coiled coil myosin like BCL2 interacting protein|Coiled-coil myosin-like BCL2-interacting protein|GT197|Protein GT197|VPS 30|VPS30
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Becn1 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
BECN1
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, SH-SY5Y whole cell lysate; All lanes: BECN1 antibody at 4ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 52 kDa; Observed band size: 52 kDa
IHC image diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC07912 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).