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Antibodies

Hsp90Ab1 Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC07147-50UG
100 ug
£454.00
CAC07147-100UG
About this Product
SKU:
CAC07147
Additional Names:
90 kda heat shock protein beta HSP90 beta|beta|D6S182|FLJ26984|Heat shock 84 kDa|Heat shock 90kD protein 1|Heat shock 90kDa protein 1 beta|Heat shock protein 90 alpha family class B member 1|Heat shock protein 90 kDa|Heat shock protein 90kDa alpha (cytosolic) class B member 1|Heat shock protein 90kDa alpha family class B member 1|Heat shock protein beta|Heat shock protein HSP 90 beta|Heat shock protein HSP 90-beta|HS90B_HUMAN|HSP 84|HSP 90|HSP 90 b|HSP 90b|HSP84|HSP90 BETA|hsp90ab1|HSP90B|HSPC2|HSPCB
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Hsp90Ab1 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
HSP90AB1
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate; All lanes: HSP90AB1 antibody at 2ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 84 kDa; Observed band size: 84 kDa
IHC image diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CAC07147 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).