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Antibodies

Ctnnb1 Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC07113-50UG
100 ug
£454.00
CAC07113-100UG
About this Product
SKU:
CAC07113
Additional Names:
88kDa|b-catenin|beta 1|Beta catenin|Beta-catenin|Cadherin associated protein|Catenin (cadherin associated protein)|Catenin beta 1|Catenin beta-1|CATNB|CHBCAT|CTNB1_HUMAN|CTNNB|CTNNB1|DKFZp686D02253|FLJ25606|FLJ37923|OTTHUMP00000162082|OTTHUMP00000165222|OTTHUMP00000165223|OTTHUMP00000209288|OTTHUMP00000209289
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Ctnnb1 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF, IP.
Host:
Rabbit
Immunogen:
CTNNB1
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate; All lanes: CTNNB1 antibody at 2.5ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 86 kDa; Observed band size: 86 kDa
Immunohistochemistry of paraffin-embedded human colon cancer using CAC07113 at dilution of 1:100
IHC image diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC07113 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).