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Antibodies

Atg3 Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC07035-50UG
100 ug
£454.00
CAC07035-100UG
About this Product
SKU:
CAC07035
Additional Names:
2610016C12Rik|Apg 3|APG3|APG3 autophagy 3 like|APG3 like|APG3-like|APG3L|Apg3p|ATG 3|ATG3|ATG3 autophagy related 3 homolog|ATG3 autophagy related 3 homolog (S. cerevisiae)|ATG3_HUMAN|Autophagy 3|Autophagy Apg3p/Aut1p like|autophagy related 3|Autophagy related protein 3|Autophagy-related protein 3|DKFZp564M1178|FLJ22125|hApg3|homolog of|MGC15201|OTTHUMP00000214547|OTTHUMP00000214548|PC3 96|Protein PC3-96|S. cerevisiae|Ubiquitin-like-conjugating enzyme ATG3
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Atg3 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
ATG3
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate; All lanes: ATG3 antibody at 4ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 36 kDa; Observed band size: 36 kDa
IHC image diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:1200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC07035 at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).