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Antibodies

Actb Polyclonal Antibody

Product Sizes
50 ug
CAC07011-50UG
100 ug
CAC07011-100UG
About this Product
SKU:
CAC07011
Additional Names:
A26C1A|A26C1B|ACTB|ACTB_HUMAN|Actin|Actin beta|Actin cytoplasmic 1|Actx|b actin|b-actin|Beta cytoskeletal actin|Beta-actin|BRWS1|cytoplasmic 1|E430023M04Rik|Melanoma X actin|MGC128179|N-terminally processed|PS1TP5 binding protein 1|PS1TP5BP1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Actb Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
ACTB
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Western Blot; Positive WB detected in: HepG2 whole cell lysate; All lanes: ACTB antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 42 kDa; Observed band size: 42 kDa
Immunohistochemistry of paraffin-embedded human endometrial cancer using CAC07011 at dilution of 1:100
IHC image diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.