botA Recombinant Protein
Product Sizes
20ug
OPCA02868-20UG
100ug
OPCA02868-100UG
About this Product
- SKU:
- OPCA02868
- Additional Names:
- Bontoxilysin-A;botulinum neurotoxin type A;Botulinum neurotoxin type A1;CBO_RS04150;CBO0806.
- Extra Details:
- Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system; with frequent heart or respiratory failure (PubMed:15394302; PubMed:7578132). Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release; when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol (PubMed:17666397; PubMed:19096517). Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles; preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release. Toxin activity requires polysialylated gangliosides; GT1b supports activity better than GD1a (PubMed:12089155). Binds to host peripheral neuronal presynaptic membranes via the synaptic vesicle glycoproteins SV2A; SV2B and SV2C (PubMed:16543415). It binds directly to the largest lumenal (intravesicular) loop of SV2A; SV2B and SV2C that is transiently exposed outside of cells during exocytosis; gangliosides enhance binding (PubMed:16543415; PubMed:16545378; PubMed:18815274). Recognizes an N-linked glycan on SV2 proteins (PubMed:18815274; PubMed:27294781). May also use FGFR3 as a receptor (PubMed:23696738). Toxin uptake into neural cells requires stimulation (incubation with K(+) to stimulate receptor exposure) to be internalized by receptor-mediated endocytosis (PubMed:16543415; PubMed:19650874; PubMed:21632541; PubMed:21832053). Subsequently the toxin colocalizes with its receptor in host cells (PubMed:16543415; PubMed:19650874). Toxin uptake can be blocked by the appropriate SV2 protein fragments in cell culture (PubMed:16543415).
- Gene Details:
- botulinum neurotoxin type A
- Molecular Weight:
- 52 kDa
- Protein Details:
- Botulinum neurotoxin type A
- Purity:
- Greater than 90% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Source:
- Yeast
- Storage Conditions:
- -20Â[o]C or -80Â[o]C
- Supplier:
- Aviva Systems Biology
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
- Manufacturer's Data Sheet:html_datasheet.php
