Anti-NFM (Neurofilament Medium Chain) Antibody
Product Sizes
200 ul
NFM-200UL
About this Product
- SKU:
- NFM
- Additional Names:
- NEFM NEF3 NFM|Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
- Application:
- Immunocytochemistry, Immunohistochemistry, Western Blot
- Buffer:
- Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preserva
- CE/IVD:
- RUO
- Clonality:
- Polyclonal
- Concentration:
- 2 mg/ml
- Extra Details:
- NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. After several boosts, hens were co-boosted with recombinant NFM protein expressed in bacteria. These antibodies have been validated with human, mouse and rat tissues.
- Formulation:
- Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preserva
- Host:
- Chicken/Avian
- Immunogen:
- Recombinant construct containing the C-terminus of the human sequence expressed in Escherichia coli
- Isotype:
- IgY
- Molecular Weight:
- 150 kDa
- Physical State:
- Liquid
- Reactivities:
- Human, Mouse, Rat
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 2-8[o]C Aliquot. Avoid freeze/thaw cycles. Protect from light.
- Type:
- Antibody: Polyclonal Antibody
