Anti-NFM (Neurofilament Medium Chain) Antibody
Product Sizes
200 ul
£352.00
NFM-200UL
About this Product
- SKU:
- NFM
- Additional Names:
- Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
- Application:
- Immunocytochemistry, Immunohistochemistry, Western Blot
- Buffer:
- Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS"
- CE/IVD:
- RUO
- Clonality:
- Polyclonal
- Concentration:
- 2 mg/ml
- Extra Details:
- NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. After several boosts, hens were co-boosted with recombinant NFM protein expressed in bacteria. These antibodies have been validated with human, mouse and rat tissues., Aves Labs
- Format:
- IgY Fraction
- Formulation:
- Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preserva
- Host:
- Chicken/Avian
- Immunogen:
- Recombinant construct containing the C-terminus of the human sequence expressed in Escherichia coli
- Isotype:
- IgY
- Molecular Weight:
- 150 kDa
- Physical State:
- Liquid
- Purification:
- Chickens were immunized with NFM protein was purified from bovine spinal cords. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.
- Reactivities:
- Human, Mouse, Rat
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 2-8[o]C Protect from light.
- Type:
- Antibody: Polyclonal Antibody
