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Antibodies

anti-CD19 antibody [SQab1869]

Product Sizes
50 ul
£349.00
ARG66321-50UL
100 ul
£477.00
ARG66321-100UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG66321
Additional Names:
Differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD antigen CD19; CVID3
Application:
Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
CE/IVD:
RUO
translate.label.attr.clone:
SQab1869
Clonality:
Monoclonal
Extra Details:
CD19: Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
Host:
Rabbit
Immunogen:
Synthetic peptide around the C-terminus of CD19.
Isotype:
IgG
Purification:
Protein A Purified; Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Flow Cytometry: Ramos cells were fixed with 4% paraformaldehyde for 10 mins. The cells were stained with ARG66321 anti-CD19 antibody [SQab1869] (blue) at 1:10 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.
Immunofluorescence: Raji cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66321 anti-CD19 antibody [SQab1869] (green) at 1:50 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemistry: Formalin-fixed and paraffin-embedded spleen tissue stained with ARG66321 anti-CD19 antibody [SQab1869] at 1:400 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
Immunoprecipitation: 0.4 mg of Ramos lysate immunoprecipitated (1:50) and stained with ARG66321 anti-CD19 antibody [SQab1869]. 1) ARG66321 IP in Ramos whole cell lysate, 2) Rabbit IgG instead of ARG66321 in Ramos whole cell lysate, and 3) Ramos whole cell lysate, 10 ug (input).
Western blot: 10 ug of Ramos, Daudi and Raji cell lysates stained with ARG66321 anti-CD19 antibody [SQab1869] at 1:2000 dilution.