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Antibodies

anti-Cytokeratin 20 antibody [SQab1737]

Product Sizes
50 ul
£349.00
ARG66248-50UL
100 ul
£477.00
ARG66248-100UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG66248
Additional Names:
KRT21; CK20; K20; CD20; CK-20; Keratin-20; Cytokeratin-20; Keratin, type I cytoskeletal 20; Protein IT
Application:
Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
CE/IVD:
RUO
translate.label.attr.clone:
SQab1737
Clonality:
Monoclonal
Extra Details:
Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
Host:
Rabbit
Immunogen:
Synthetic peptide around the C-terminus of Human Cytokeratin 20.
Isotype:
IgG
Purification:
Protein A Purified; Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Flow Cytometry: HT-29 cells stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the primary antibody at 1:1000 dilution in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat anti-rabbit Alexa Fluor® 488 (IgG H+L) at 1:2000 dilution for 20 min at 4°C. Unlabelled sample (Blue) was used as a control.
Immunofluorescence: HT-29 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for half an hour at RT. Samples were stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] (green) at 1:200, 4°C.
Immunohistochemistry: Formalin-fixed and paraffin-embedded colonic adenocarcinoma stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:800. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
Immunoprecipitation: Cytokeratin 20 was immunoprecipitated from 0.5 mg of HT-29 lysate with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:50 dilution.
Western blot: 10 ug of HCT-116 and HT-29 cell lysates stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:5000 dilution.