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Antibodies

anti-CD20 antibody [SQab1719]

Product Sizes
50 ul
£349.00
ARG66197-50UL
100 ul
£477.00
ARG66197-100UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG66197
Additional Names:
Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A1
Application:
Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Buffer:
PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
CE/IVD:
RUO
translate.label.attr.clone:
SQab1719
Clonality:
Monoclonal
Extra Details:
CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Host:
Rabbit
Immunogen:
Synthetic peptide around the C-terminus of CD20.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Flow Cytometry: Raji cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG66197 anti-CD20 antibody [SQab1719] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
Immunofluorescence: Raji cells fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG66197 anti-CD20 antibody [SQab1719] (green) at 1:1000 at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human appendix tissue stained with ARG66197 anti-CD20 antibody [SQab1719] at 1:20000 dilution.
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human spleen tissue stained with ARG66197 anti-CD20 antibody [SQab1719] at 1:20000 dilution.
Immunoprecipitation: 0.4 mg of Raji whole cell lysate was immunoprecipitated (1:20 dilution) and stained with ARG66197 anti-CD20 antibody [SQab1719].