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Antibodies

anti-ApoJ / Clusterin antibody

Product Sizes
100 ug
ARG63834-100UG
Academic Pricing
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About this Product
SKU:
ARG63834
Additional Names:
Clusterin; Apolipoprotein J; SGP-2; SP-40; APOJ; ApoJbeta; CLU2; CLU1; NA1/NA2; TRPM2; CLI; SGP2; Apo-J; TRPM-2; ApoJalpha; Aging-associated gene 4 protein; APO-J; Complement cytolysis inhibitor a chain; Ku70-binding protein 1; Complement cytolysis inhibitor b chain; Testosterone-repressed prostate message 2; Complement-associated protein SP-40,40; KUB1; AAG4; Complement cytolysis inhibitor
Application:
IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.5 mg/ml
Extra Details:
The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]
Host:
Goat
Immunogen:
C-EKALQEYRKKHREE
Isotype:
IgG
Purification:
Purified from goat serum by antigen affinity chromatography.
Reactivities:
Canine, Human
Shipping Conditions:
Blue Ice
Specificity:
This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63834 anti-ApoJ / Clusterin antibody (green) at 10 ug/ml for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 ug/ml dilution.
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63834 anti-ApoJ / Clusterin antibody at 2.5 ug/ml dilution followed by AP-staining.
Western blot: 35 ug of Human colon lysate (in RIPA buffer) stained with ARG63834 anti-ApoJ / Clusterin antibody at 0.1 ug/ml dilution and incubated at RT for 1 hour.