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Antibodies

anti-COX2 antibody

Product Sizes
100 ug
£616.00
ARG63562-100UG
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG63562
Additional Names:
PHS II; Prostaglandin H2 synthase 2; PHS-2; Cyclooxygenase-2; PGHS-2; COX2; PGG/HS; COX-2; GRIPGHS; hCox-2; PGH synthase 2; Prostaglandin G/H synthase 2; Prostaglandin-endoperoxide synthase 2; EC 1.14.99.1
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.5 mg/ml
Extra Details:
COX2: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
Host:
Goat
Immunogen:
C-NPTVLLKERSTEL
Isotype:
IgG
Purification:
Purified from goat serum by antigen affinity chromatography.
Reactivities:
Bovine, Canine, Human, Mouse, Porcine
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Western blot: 35 ug of H460 cell lysate (in RIPA buffer) stained with ARG63562 anti-COX2 antibody at 0.5 ug/ml dilution and incubated at RT for 1 hour.
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63562 anti-COX2 antibody (blue line) at 10 ug/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63562 anti-COX2 antibody (green) at 10 ug/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 ug/ml dilution.
Immunofluorescence: Paraformaldehyde fixed NIH/3T3 cells permeabilized with 0.15% Triton. Cells were stained with ARG63562 anti-COX2 antibody (green) at 10 ug/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 ug/ml dilution.
Western blot: 35 ug of A549 (A) and Daudi (B) cell lysates (in RIPA buffer) stained with ARG63562 anti-COX2 antibody at 0.1 ug/ml dilution and incubated at RT for 1 hour.