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Antibodies

anti-IL6 antibody [1.2-2B11-2G10]

Product Sizes
100 ug
£397.00
ARG56591-100UG
About this Product
SKU:
ARG56591
Additional Names:
B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS (pH 7.2)
CE/IVD:
RUO
translate.label.attr.clone:
1.2-2B11-2G10
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
Host:
Mouse
Immunogen:
E.coli derived Recombinant Human IL-6.(PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM)
Isotype:
IgG1, kappa
Purification:
Protein A Purified; Affinity Purified
Reactivities:
Human, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56591 anti-IL6 antibody [1.2-2B11-2G10] concentration is 0.125 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
Western blot: 250 - 0.24 ng (left to right) of Human IL-6 stained with ARG56591 anti-IL6 antibody [1.2-2B11-2G10], under non-reducing conditions.
Western blot: 250 - 0.24 ng (left to right) of Human IL-6 stained with ARG56591 anti-IL6 antibody [1.2-2B11-2G10], under reducing conditions.
Sandwich ELISA: ARG56591 anti-IL6 antibody [1.2-2B11-2G10] as a capture antibody at 1 - 2 ug/ml dilution combined with ARG56736 anti-IL6 antibody (Biotin) as a detection antibody at 0.1 - 0.2 ug/ml dilution. Results of a typical standard run with optical density.