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Antibodies

anti-UCHL1 / PGP9.5 antibody [BH7]

Product Sizes
50 ul
£456.00
ARG52465-50UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG52465
Additional Names:
PGP95; UCH-L1; PGP9.5; PARK5; Ubiquitin thioesterase L1; HEL-117; Neuron cytoplasmic protein 9.5; Uch-L1; EC 6.-.-.-; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; NDGOA; EC 3.4.19.12
Application:
IHC-Frozen, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
Total IgG fraction and 10 mM Sodium azide
CE/IVD:
RUO
translate.label.attr.clone:
BH7
Clonality:
Monoclonal
Extra Details:
Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 . It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein . The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice . Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease . Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.
Host:
Mouse
Immunogen:
Recombinant full length human UCHL1 purified from E. coli
Isotype:
IgG1
Reactivities:
Bovine, Human, Mouse, Other Mammal, Porcine, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Immunofluorescence: Cortical neuron-glial culture from E20 Rat stained with ARG52465 anti-UCHL1 / PGP9.5 antibody [BH7] (green) at 1:5000 dilution, and costained with anti-GFAP antibody (red) at 1:5000 dilution. DAPI (blue) for nuclear staining.
Immunohistochemistry: Rat spinal cord stained with ARG52465 anti-UCHL1 / PGP9.5 antibody [BH7] (red) and ARG52347 anti-Neurofilament NF-H antibody (green). The large cells are A Alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.
Immunohistochemistry: Frozen section of Rat hippocampal tissue stained with ARG52465 anti-UCHL1 / PGP9.5 antibody [BH7] (green) at 1:5000 dilution, and costained with ARG10712 anti-FOX3 / NeuN antibody (red) at 1:2000 dilution. DAPI (blue) for nuclear staining. Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies.
Western blot: Rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein stained with ARG52465 anti-UCHL1 / PGP9.5 antibody [BH7].
Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord, Pig brain and Pig spinal cord lysates stained with ARG52465 anti-UCHL1 / PGP9.5 antibody [BH7] (green) at 1:10000 dilution.