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Antibodies

anti-TDP43 antibody [3H8]

Product Sizes
50 ul
£408.00
ARG10763-50UL
Academic Pricing
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About this Product
SKU:
ARG10763
Additional Names:
TAR DNA-binding protein 43; TDP-43; ALS10
Application:
IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS and 50% Glycerol.
CE/IVD:
RUO
translate.label.attr.clone:
3H8
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]
Host:
Mouse
Immunogen:
Full length recombinant Human TDP43 expressed in and purified from E. coli.
Isotype:
IgG1
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry: Frozen sections of Formalin-fixed adult Rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained with ARG10763 anti-TDP43 antibody [3H8]. Chicken antibody to GFAP (green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDP antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TDP43 and blue due to their content of DNA, stained blue with DAPI.
Immunohistochemistry: Frozen section of Rat hippocampus stained with ARG10763 anti-TDP43 antibody [3H8] (red) at 1:2000 dilution and costained with ARG52313 anti-GFAP antibody (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies.)
Western blot: Crude extract of Mouse brain nuclear fraction (left) and cytoplasmic fraction (right) stained with ARG10763 anti-TDP43 antibody [3H8]. There is a strong clear band in the nuclear preparation running at 43 kDa.
Western blot: Rat brain (whole), Rat brain (nuclear), Mouse brain (whole), Mouse brain (nuclear). The blots were stained with ARG10763 anti-TDP43 antibody [3H8] (green) at 1:2000 dilution.