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Antibodies

anti-Hsp 27 antibody

Product Sizes
50 ul
£408.00
ARG10684-50UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG10684
Additional Names:
HSP 27; Heat shock 27 kDa protein; HMN2B; HS.76067; SRP27; HEL-S-102; HspB1; CMT2F; 28 kDa heat shock protein; HSP27; Heat shock protein beta-1; Hsp25; Estrogen-regulated 24 kDa protein; Stress-responsive protein 27; HSP28
Application:
IHC-Frozen, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS and 0.02% Sodium azide.
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
Host:
Chicken/Avian
Immunogen:
Recombinant full length purified HSP27 from E. coli.
Isotype:
IgY
Reactivities:
Avian, Bovine, Horse, Human, Non-human Primate, Porcine
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Immunocytochemistry: HeLa cells stained with ARG10684 anti-Hsp 27 antibody (green), and co-stained with a monoclonal antibody to High mobility Group B protein 1 (HMGB1, red) and DNA (blue). ARG10684 reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins, while the HMGB1 antibody reveals strong nuclear staining which overlaps with the DNA staining.
Immunofluorescence: HeLa cells stained with ARG10684 anti-Hsp 27 antibody (green) at 1:1000 dilution and costained with Mouse mAb to Hsp 60 (red) at 1:5000 dilution. DAPI (blue) for nuclear staining.
Western blot: HeLa cell crude extracts stained with ARG10684 anti-Hsp 27 antibody.
Western blot: NIH/3T3, C6, HeLa, HEK293, SH-SY5Y and COS-1 cell lysates stained with ARG10684 anti-Hsp 27 antibody (red) at 1:2000 dilution. The blot was simultaneously stained with Mouse mAb to beta tubulin (green) at 1:10000 dilution.