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Assay Kits & ELISAs

Intracellular ROS Assay Kit (Fluorometric)

Product Sizes
96 assays
ARG81192-96ASSAYS
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG81192
Application:
Cell-based/Functional Assay
CE/IVD:
RUO
Extra Details:
In chemistry and biology, reactive oxygen species (ROS) are highly reactive chemicals formed from diatomic oxygen (O2), water, and hydrogen peroxide. Some prominent ROS are hydroperoxide (O2H), superoxide (O2-), hydroxyl radical (OH.), and singlet oxygen. ROS are pervasive because they are readily produced from O2, which is abundant. ROS are important in many ways, both beneficial and otherwise. ROS function as signals, that turn on and off biological functions. They are intermediates in the redox behavior of O2, which is central to fuel cells. ROS are central to the photodegradation of organic pollutants in the atmosphere. Most often however, ROS are discussed in a biological context, ranging from their effects on aging and their role in causing dangerous genetic mutations.[Wikipedia]
Immunogen:
ROS
Physical State:
Cell Culture
Reactivities:
Wide/Non-species Specific
Sensitivity:
0.01 nM
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C, -20[o]C
Supplier:
Arigo Biolaboratories
Type:
Assay Kits: Detection
ARG81192 Intracellular ROS Assay Kit (Fluorometric) results of a typical standard run with optical density reading at 480 nm excitation / 530 nm emission.
DCF Fluorescence in H2O2 treated HeLa cells after 1 hour. Left: 0 uM; Right: 1000 uM.
50,000 HeLa cells in a 96-well plate were first pretreated with 1 mM DCFH-DA for 60 minutes at 37°C. Cells were then treated with various concentrations of H2O2 for 20 minutes.