Lba Cas12a (Cpf1) Nuclease
Product Sizes
20 ul
£177.00
YXX26701-20UL
200 ul
£767.00
YXX26701-200UL
About this Product
- SKU:
- YXX26701
- Additional Names:
- CRISPR-associatedendonucleaseCas12a,CRISPR-associatedendonucleaseCpf1,LbaCas12a(Cpf1),LbaCas12a,Cpf1,LbCas12a
- Extra Details:
- LbaCas12anucleaseisderivedfromLachnospiraceaebacteriumND2006andisasite-specificDNAendonucleasemediatedbyasingle-guideRNA(gRNA)withalengthof41-44nucleotides.InthepresenceofaPAM(TTTN)sequenceonthetargetdouble-strandedDNA,LbCas12aspecificallycleavesthetargetdsDNA,resultingindouble-strandbreaksandthegenerationofstickyends.Incontrast,thespecificcleavageofsingle-strandedDNAtargetsbyLbCas12adoesnotrequireaPAMsequence.\r\n LbaCas12acontainssimianvirus40(SV40)Tantigennuclearlocalizationsequences(NLS)atboththeN-terminusandC-terminus.WhenCas12aisexpressedwiththeseNLSsequences,theCas12a-RNPcomplexcanrapidlylocalizetothenucleusuponcellularentry.\r\n Additionally,bothdouble-strandedandsingle-strandedDNAtargetscanactivatethetrans-cleavageactivityofLbCas12a.Specifically,uponformationofaternarycomplexamongtheLbCas12aenzyme,crRNA,andtargetDNA,theenzymebecomesactivatedandexhibitsnon-specifictrans-cleavageactivity,degradinganysingle-strandedDNA(ssDNA)presentinthesystem.Consequently,LbCas12acannotonlybeusedforspecificcleavageofdsDNAinvitrobutalsoenablesrapiddetectionoftargetnucleicacids.
- Physical State:
- Liquid
- Purity:
- >90% as determined by sds-page.
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles.
- Supplier:
- AntibodySystem
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
