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Proteins and Peptides

SARS S protein (R667A, K968P, V969P), His Tag (MALS verified)

Product Sizes
100 ug
£560.00
SPN-S52H6-100UG
1 mg
£4563.00
SPN-S52H6-1MG
About this Product
SKU:
SPN-S52H6
Additional Names:
S glycoprotein|S protein|Spike|Spike glycoprotein
Buffer:
50 mM Tris, 150 mM NaCl, 0.2 M Sodium citrate, pH5.5
Extra Details:
SARS S protein (R667A, K968P, V969P), His Tag (SPN-S52H6) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.
Immunogen:
Spike protein
Molecular Weight:
136.3 kDa
Physical State:
Powder
Shipping Conditions:
Ambient
Storage Conditions:
-20[o]C
Supplier:
ACRO Biosystems
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SARS S protein (R667A, K968P, V969P), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) at 2 ug/mL (100 uL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL (QC tested).
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 ug/mL (100 uL/well) can bind SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) with a linear range of 2-20 ng/mL (Routinely tested).