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Proteins and Peptides

MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (MALS verified)

Product Sizes
50 ug
£471.00
SPN-M52H5-50UG
500 ug
£2985.00
SPN-M52H5-500UG
About this Product
SKU:
SPN-M52H5
Buffer:
0.1 M Sodium citrate, pH5.5
Extra Details:
MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (SPN-M52H5) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Trp 1295 (Accession # K0BRG7 (R748A, R751A, V1060P, L1061P)). The recombinant protein has a T4 fibritin trimerization motif. Proline substitutions (V1060P, L1061P) and alanine substitutions (R748A, R751A) are introduced to stabilize the trimeric prefusion state of MERS-CoV Spike protein and abolish the furin cleavage site, respectively.
Immunogen:
Spike protein
Molecular Weight:
145.9 kDa
Physical State:
Powder
Shipping Conditions:
Ambient
Storage Conditions:
-20[o]C
Supplier:
ACRO Biosystems
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human DPPIV, Fc Tag (Cat. No. DP4-H5266) at 5 ug/mL (100 uL/well) can bind MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H5) with a linear range of 0.1-3 ng/mL (QC tested).
Immobilized MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H5) at 5 ug/mL (100 uL/well) can bind Human DPPIV, Fc Tag (Cat. No. DP4-H5266) with a linear range of 0.1-2 ng/mL (Routinely tested).