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Biotinylated SARS-CoV-2 Spike Trimer Protein, His,Avitag[TM] (BA.4/Omicron) (MALS verified)

Product Sizes
25 ug
£583.00
SPN-C82ET-25UG
200 ug
£2573.00
SPN-C82ET-200UG
About this Product
SKU:
SPN-C82ET
Additional Names:
S glycoprotein|S protein|Spike|Spike glycoprotein
Buffer:
0.1 M Sodium citrate, pH5.5
Conjugate:
Biotin
Extra Details:
Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (BA.4/Omicron) (SPN-C82Et) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (T19I, LPP24-26del, A27S, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N658S, N679K, P681H, N764K, D796Y, Q954H, N969K, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.4; GISAID clade: GRA). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Immunogen:
Spike protein
Molecular Weight:
139.4 kDa
Physical State:
Powder
Shipping Conditions:
Ambient
Storage Conditions:
-20[o]C
Supplier:
ACRO Biosystems
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (BA.4/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (BA.4/Omicron) (Cat. No. SPN-C82Et) at 1 ug/mL (100 uL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 ug/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.001-0.013 ug/mL (QC tested).
Immobilized Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (BA.4/Omicron) (Cat. No. SPN-C82Et) at 1 ug/mL (100 uL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 ug/well) plate can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M130) with a linear range of 0.1-2 ng/mL (Routinely tested).