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Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag[TM], Super stable trimer (MALS verified)

Product Sizes
25 ug
£583.00
SPN-C82E3-25UG
200 ug
£2573.00
SPN-C82E3-200UG
About this Product
SKU:
SPN-C82E3
Additional Names:
S glycoprotein|S protein|Spike|Spike glycoprotein
Buffer:
0.1 M Sodium citrate, pH5.5
Conjugate:
Biotin
Extra Details:
Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer (SPN-C82E3) is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (D614G)) and D614G mutation, which has become increasingly common in SARS-CoV-2 viruses from around the world . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Immunogen:
Spike protein
Molecular Weight:
139.7 kDa
Physical State:
Powder
Shipping Conditions:
Ambient
Storage Conditions:
-20[o]C
Supplier:
ACRO Biosystems
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 ug/mL (100 uL/well) can bind Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer (Cat. No. SPN-C82E3) with a linear range of 2-63 ng/mL (QC tested).
Immobilized Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer (Cat. No. SPN-C82E3) at 1 ug/mL (100 uL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5 ug/well) plate, can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.1-2 ng/mL (Routinely tested).