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Biotinylated Human Complement C5 Protein, His,Avitag[TM] (MALS verified)

Product Sizes
25 ug
£377.00
CO5-H82E9-25UG
200 ug
£1466.00
CO5-H82E9-200UG
About this Product
SKU:
CO5-H82E9
Buffer:
PBS
Conjugate:
Biotin
Extra Details:
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Immunogen:
Complement C5
Molecular Weight:
73.9 kDa (B Beta chain) & 116.1 kDa (A Alpha chain)
Physical State:
Powder
Shipping Conditions:
Ambient
Storage Conditions:
-20[o]C
Supplier:
ACRO Biosystems
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
Biotinylated Human Complement C5, His,Avitag on SDS-PAGE under reducing (R) and under non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Biotinylated Human Complement C5, His,Avitag (Cat. No. CO5-H82E9) at 1 ug/mL (100 uL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 ug/well) plate can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
Immobilized Eculizumab Biosimilar at 2 ug/mL (100 uL/well) can bind Biotinylated Human Complement C5, His,Avitag (Cat. No. CO5-H82E9) with a linear range of 1-63 ng/mL (Routinely tested).