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Proteins and Peptides

Biotinylated Human CD73 / NT5E Protein, His,Avitag[TM]

Product Sizes
25 ug
£377.00
CD3-H82E3-25UG
200 ug
£1614.00
CD3-H82E3-200UG
About this Product
SKU:
CD3-H82E3
Additional Names:
5'-NT|5'-Nucleotidase|CD73|NT5|NT5E|NTE
Buffer:
20 mM Tris, 120 mM NaCl, pH7.5
Conjugate:
Biotin
Extra Details:
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Immunogen:
CD73
Molecular Weight:
61.7 kDa
Physical State:
Powder
Shipping Conditions:
Ambient
Storage Conditions:
-20[o]C
Supplier:
ACRO Biosystems
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Biotinylated Human CD73, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Monoclonal Anti-Human CD73 Antibody, Human IgG1 at 5 ug/mL (100 uL/well) can bind Biotinylated Human CD73, His,Avitag (Cat. No. CD3-H82E3) with a linear range of 0.6-5 ng/mL (QC tested).
Immobilized Biotinylated Human CD73, His,Avitag (Cat. No. CD3-H82E3) at 1 ug/mL (100 uL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 ug/well) plate, can bind Monoclonal Anti-Human CD73 Antibody, Human IgG1 with a linear range of 0.3-2 ng/mL (Routinely tested).