Anti-p21 protein [Y13-259]
Product Sizes
1 mg
AB03219-6-1-BT-1MG
About this Product
- SKU:
- AB03219-6-1-BT
- Additional Names:
- p21 ras protein; Ras; p21ras
- Application:
- Block/Inhibit/Neutralise, Immunohistochemistry, Immunoprecipitation, Western Blot
- Buffer:
- PBS
- translate.label.attr.clone:
- Y13-259
- Clonality:
- Recombinant Monoclonal
- Formulation:
- PBS only.
- Host:
- Rat
- Immunogen:
- The original antibody was generated by immunizing rats with Ha-MuSV-transformed NRK cells. Screening of sera of individual animals was carried out to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Human, Mouse, Rat
- Shipping Conditions:
- Blue Ice
- Specificity:
- The antibody binds to an epitope common to all p21ras isoforms (Glu63-Arg73), a region known as loop 4. The antibody broadly reacts with ras proteins, including M-Ras and N-Ras.
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab03219-6.1-BT
