Anti-MOG [M3-31]
Product Sizes
1 mg
AB01208-10-0-BT-1MG
About this Product
- SKU:
- AB01208-10-0-BT
- Additional Names:
- Myelin oligodendrocyte glycoprotein; rMOG; hMOG
- Application:
- Block/Inhibit/Neutralise, ELISA, Immunohistochemistry
- Buffer:
- PBS
- translate.label.attr.clone:
- M3-31
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric human antibody was made using the variable domain sequences of the original Marmoset Fab format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS only.
- Host:
- Human
- Immunogen:
- Callithrix jacchus marmosets were used, and experimental allergic encephalomyelitis was induced by the injection of rat MOG (aa1-125) into the marmosettes. The rMOG was expressed in E. coli and purified to homogeneity. The animals were killed 4-70 days after the onset of symptoms of EAE. Bone marrow and spleen cells were obtained from an immunized C. jacchus, the RNA extracted with Trizol reagent and rtPCR used to generate cloning inserts containing Fab portions of IgGk. Phage display was then used to select for the MOG-reactive Fab fragments using the pCOMB3H phage display vector, and binding confirmed using an ELISA.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Human, Rat
- Shipping Conditions:
- Blue Ice
- Specificity:
- Is displaced by M38 and M45 and is weakly displaced by M26 although this is not mutual which may suggest the M26-mediated displacement is conformationally driven, although this has not been confirmed. The epitope recognised is a structural epitope, shown by the lack of recognition of linear MOG peptides. Binds MOG expressed in CHO cells.
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab01208-10.0-BT
