Anti-MOG [M3-8]
Product Sizes
200 ug
AB01206-10-3-200UG
About this Product
- SKU:
- AB01206-10-3
- Additional Names:
- Myelin oligodendrocyte glycoprotein; rMOG
- Application:
- Block/Inhibit/Neutralise, ELISA, Immunohistochemistry
- Buffer:
- PBS with 0.02% Proclin 300.
- translate.label.attr.clone:
- M3-8
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric human antibody was made using the variable domain sequences of the original Marmoset Fab format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS with 0.02% Proclin 300.
- Host:
- Human
- Immunogen:
- Callithrix jacchus marmosets were used, and experimental allergic encephalomyelitis was induced by the injection of rat MOG (aa1-125) into the marmosettes. The rMOG was expressed in E. coli and purified to homogeneity. The animals were killed 4-70 days after the onset of symptoms of EAE. Bone marrow and spleen cells were obtained from an immunized C. jacchus, the RNA extracted with Trizol reagent and rtPCR used to generate cloning inserts containing Fab portions of IgGk. Phage display was then used to select for the MOG-reactive Fab fragments using the pCOMB3H phage display vector, and binding confirmed using an ELISA.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Rat
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognises an epitope which is distinct from M26, M38, M45, M3-24 and M3-31. Is able to displace native anti-MOG Abs from C. jacchus serum. Has been shown to bind in situ by immunofluorescence. The epitope recognised is a structural epitope, shown by the lack of recognition of linear MOG peptides. Has also been shown to compete with anti-MOG Abs from three patients with MS. Does not bind to MOG ex
- Storage Conditions:
- 2-8[o]C Aliquot., -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab01206-10.3
