Anti-Endothelial Progenitor Cell [UG1]
Product Sizes
200 ug
AB01195-23-0-200UG
About this Product
- SKU:
- AB01195-23-0
- Additional Names:
- gUG1; colony-forming unit Hill cells; CFU-Hills; circulating angiogenic cells; CACs; endothelial colony-forming cells; ECFCs; early outgrowth cells; early EPCs; late outgrowth cells; late EPCs
- Application:
- Flow Cytometry
- Buffer:
- PBS with 0.02% Proclin 300.
- translate.label.attr.clone:
- UG1
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS with 0.02% Proclin 300.
- Host:
- Rabbit
- Immunogen:
- Peripheral blood was obtained from human donors. The PBMCs were obtained by density centrifugation and cultured. Two ISA brown chickens were then injected intradermally and after 50 days the spleen and bone marrow was harvested. RT-PCR was performed on cells from the spleen and bone marrow which was used to generate a cDNA library. Primers were then used to amplify the VL and VH genes and this was assembled in an scFv format. Phage libraries were generated and the library was biopanned. The biopanned library was then transformed into XL1 blue bacteria and colonies chosed at random from these.
- Isotype:
- IgG
- Purification:
- Purified
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Specificity:
- UG1 binds predominantly to a subset of CD14+ monocytes that mostly co-express lymphocyte markers but UG1 does not preferentially bind to cells which express receptors for proangiogenic factors.
- Storage Conditions:
- 2-8[o]C Aliquot., -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab01195-23.0
