Anti-VEGF [G6-31]
Product Sizes
1 mg
AB01022-23-0-BT-1MG
About this Product
- SKU:
- AB01022-23-0-BT
- Additional Names:
- VEGF-A; Vascular endothelial growth factor; vascular permeability factor; VPF; MVCD1
- Application:
- Block/Inhibit/Neutralise, Crystallization, ELISA, Immunofluorescence, Surface Plasmon Resonance
- Buffer:
- PBS
- translate.label.attr.clone:
- G6-31
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS only.
- Host:
- Rabbit
- Immunogen:
- The anti-mVEGF Fab G6 underwent light chain randomisation by placing stop codons in positions 91-96 in CDR-L3 and an equimolar mix of oligonucleotides designed for mutagenesis was annealed to the mV401 stop template phagemid, followed by mutagenesis and E. coli electroporation. Binding selection was performed with mVEGF immobilised on immunoplates followed by solution-phase sorting with increasing stringency and single-point competetive ELISA to screen for high-affinity clones.
- Isotype:
- IgG
- Purification:
- Purified
- Reactivities:
- Mouse
- Shipping Conditions:
- Blue Ice
- Specificity:
- G6-31 recognises a VEGF epitope which is conserved between human and mouse VEGF and overlaps with the receptor binding surface. The KD of G6-31 binding to m-VEGF and h-VEGF is ~0.9 and 1.5 nM respectively. Alanine-scanning mutagenesis of solvent-accessible residues of h-VEGF identified 10 residues that result in >4-fold loss in binding affinity including F17, M18, Y21, Q22, Y25, I83,H86, Q89 and I
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab01022-23.0-BT
