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Antibodies

Anti-Porphyrin [13G10]

Product Sizes
200 ug
Was £463.00 £440.00
AB01004-2-0-200UG
About this Product
SKU:
AB01004-2-0
Additional Names:
Fe(ToCPP); Iron(III)-meso-alpha,alpha,alpha,beta-tetrakis-orthocarboxyphenylporphyrin
Application:
Crystallization, ELISA
Buffer:
PBS with 0.02% Proclin 300.
translate.label.attr.clone:
13G10
Clonality:
Recombinant Monoclonal
Extra Details:
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation:
PBS with 0.02% Proclin 300.
Host:
Mouse
Immunogen:
BALB/c mice were immunised by injection of iron(III)-meso-alpha,alpha,alpha,beta-tetrakis-orthocarboxyphenylporphyrin (Fe(ToCPP)) conjugated to KLH. Splenocytes were obtained from immunised mice and fused with the PA1 myeloma cell line to generate a hybridoma.
Isotype:
IgG2a
Purification:
Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Specificity:
13G10 was shown to bind Fe(ToCPP) using competitive ELISA with an affinity of 20 nM. 13G10 showed that same affinity for non-metallated Fe(ToCPP). The antibody did not bind meso-tetraphenylporphyrin, suggesting that the carboxylate groups are critical for recognition.
Storage Conditions:
2-8[o]C Aliquot., -20[o]C Aliquot. Avoid freeze/thaw cycles.
Supplier:
Absolute Antibody
Type:
Antibody: Recombinant Antibodies