Anti-C5a [Fab 103]
Product Sizes
200 ug
AB00990-10-0-200UG
About this Product
- SKU:
- AB00990-10-0
- Additional Names:
- Complement Component 5a
- Application:
- Block/Inhibit/Neutralise, ELISA
- Buffer:
- PBS with 0.02% Proclin 300.
- translate.label.attr.clone:
- Fab 103
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric human antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS with 0.02% Proclin 300.
- Host:
- Human
- Immunogen:
- A panel of mAbs against the activated complement component C5a was obtained from a filamentous phage M13-Fab display library generated from mice immunized with human rC5a. Fabs isolated from the library after iterative selection against rC5a bound to both rC5a and purified C5. To isolate Fabs specific for neoepitopes expressed on C5a but not on the native complement component C5, the library was rescreened in a competitive manner. The phage Fab library was first incubated with immobilized C5 to deplete C5 reactive Fabs. The C5 nonadherent phage were then incubated with immobilized rC5a in the presence of soluble C5. Bound phage were eluted and subjected to two additional cycles of subtraction with immobilized C5 and selection with immobilized rC5a in the presence of soluble C5. After three cycles of this competitive biopanning, Fabs reactive with rC5a were isolated.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Specificity:
- Fab 103 binds only to C5a and not to C5. This antibody is likely to recognise a conformational epitope because it failed to bind C5a when it was linked to microtiter plates. The epitope is outside the active site of C5a.
- Storage Conditions:
- 2-8[o]C Aliquot., -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00990-10.0
