Anti-Potato cyst nematode [MGR48]
Product Sizes
200 ug
AB00961-23-0-200UG
About this Product
- SKU:
- AB00961-23-0
- Additional Names:
- G. rostochiensis; Globodera rostochiensis; G. rostochiensis; MRG 48; MGR-48
- Application:
- Immunofluorescence, Immunoprecipitation, Western Blot
- Buffer:
- PBS with 0.02% Proclin 300.
- translate.label.attr.clone:
- MGR48
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS with 0.02% Proclin 300.
- Host:
- Rabbit
- Immunogen:
- Sixteen mice were immunised intraperitoneally with isolated protein fractions from homogenised preparasitic second-stage juveniles of G. rostochiensis, which were mixed 1:1 with Freund's incomplete adjuvant. weeks later antiserum samples were collected for immunofluorescence microscopy. One mouse that was selected for monoclonal antibody production received a final booster injection 12 weeks after the first immunization. Three days later the mouse was sacrificed, and MAb-producing hybridoma cell lines were obtained by fusing spleen cells with SP 2/0 myeloma cells.
- Isotype:
- IgG
- Purification:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- MGR48 binds to proteins localised within the secretory granules of the subventral gland in G. rostochiensis, and has also been found to react with the subventral glands of H. glycines. MGR48 has been shown to recognise six subventral gland proteins (svp3Q, svp31a, svp3\i, svp32, svp39 and svp49) by Western blotting.
- Storage Conditions:
- 2-8[o]C Aliquot., -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00961-23.0
