Anti-IL-10 [9D7]
Product Sizes
1 mg
AB00959-13-12-BT-1MG
About this Product
- SKU:
- AB00959-13-12-BT
- Additional Names:
- Human cytokine synthesis inhibitory factor;GVHDS; IL10; Interleukin 10; Interleukin-10; IL10A; TGIF; JES3-9D7; JES3 9D7
- Application:
- Block/Inhibit/Neutralise, Crystallization, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
- Buffer:
- PBS
- translate.label.attr.clone:
- 9D7
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric human antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS only.
- Host:
- Human
- Immunogen:
- Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates. IL-10 was conjugated to JES3. The mouse non-secreting myeloma fusion partner P3X63-AG8.653, is fused to immunised rat splenocytes to produce heterohybridomas secreting rat antibody. Bioassays were used to identify neutralising mAbs, and immunochemical screens were used to identify mAbs that bind the cytokine.
- Isotype:
- IgG4
- Purification:
- Purified
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Specificity:
- The six 9D7 Fab CDR loops interact with a discontinuous IL-10M1 epitope comprised of residues 71-83 and 125-137. 9D7 non-competitively antagonises IL-10R1 binding.
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00959-13.12-BT
