Anti-CD131 [BION-1]
Product Sizes
1 mg
£1962.00
AB00957-10-3-BT-1MG
About this Product
- SKU:
- AB00957-10-3-BT
- Additional Names:
- CSF2RB; CD131; IL3RB; IL-3R common beta subunit; IL5RB; colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage); SMDP5; Common Beta-Chain; CDw131; cytokine receptor common subunit beta; beta-c; CD131; Beta Common Chain
- Application:
- Block/Inhibit/Neutralise, Cell-based/Functional Assay, Crystallization, Immunohistochemistry, Immunoprecipitation
- Buffer:
- PBS
- translate.label.attr.clone:
- BION-1
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS only.
- Host:
- Human
- Immunogen:
- BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Specificity:
- Binds to domain 4 of the common beta-chain and recognises the same binding site used by IL-5, GM-CSF and IL-3, or binds to an epitope in close proximity to it. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding.
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00957-10.3-BT
