Anti-Haemoglobin [2A1]
Product Sizes
1 mg
AB00956-23-0-BT-1MG
About this Product
- SKU:
- AB00956-23-0-BT
- Additional Names:
- Hemoglobin; Hb
- Application:
- ELISA, Western Blot
- Buffer:
- PBS
- translate.label.attr.clone:
- 2A1
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS only.
- Host:
- Rabbit
- Immunogen:
- Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend's Adjuvant.
- Isotype:
- IgG
- Purification:
- Purified
- Reactivities:
- Mouse
- Shipping Conditions:
- Blue Ice
- Specificity:
- 2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb.
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00956-23.0-BT
