Anti-HLA-A2/A28 [3PF12]
Product Sizes
1 mg
AB00947-10-0-BT-1MG
About this Product
- SKU:
- AB00947-10-0-BT
- Additional Names:
- Human Leukocyte antigen; HLA-A2; HLA-A28; HLA A2; HLA A28
- Application:
- Flow Cytometry, Immunofluorescence
- Buffer:
- PBS
- translate.label.attr.clone:
- 3PF12
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS only.
- Host:
- Human
- Immunogen:
- A 57-year-old female presented with post-transfusion purpura 5 days following a blood transfusion. Serological studies with the sera of this patient identified the presence of anti-HLA-A2. A 50-ml EDTA anti-coagulated blood sample was obtained from the patient in the acute phase of her PTP, and mononuclear cells were isolated from the blood sample by layering onto a Ficoll gradient. An oligo dT primer was used to direct first strand cDNA synthesis and IgG encoded VH was amplified and an scFv library was constructed. Phage particles expressing anti-HLA A2/A28 scFvs were selected by panning.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Specificity:
- Amino acid residues 142-145 (TTKH) of the alpha2 domain of the heavy chain of the HLA class I molecules are most likely to be involved in the formation of the epitope recognised by the scfv.
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00947-10.0-BT
