Anti-Sigma1 [5C6]
Product Sizes
1 mg
AB00926-10-0-BT-1MG
About this Product
- SKU:
- AB00926-10-0-BT
- Additional Names:
- Outer capsid protein sigma1; sigma1 protein; Reovirus strain T1L sigma 1; strain T1L S1; T1L S1; type 1 haemagglutinin; type 1 HA; T1L HA
- Application:
- Block/Inhibit/Neutralise, Crystallization, Flow Cytometry, Immunofluorescence, Immunoprecipitation
- Buffer:
- PBS
- translate.label.attr.clone:
- 5C6
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS only.
- Host:
- Human
- Immunogen:
- Murine antibodies were produced by immunising CAF1 mice with reovirus strain T3D, with subsequent immunisation boosts using UV-inactivated strain T1L reovirus. Immunised mice produced antibodies recognising both T3D and T1L. Splenocytes were obtained from immunised mice and fused with the P3Ag8.653 myeloma cell line to generate hybridomas.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Virus
- Shipping Conditions:
- Blue Ice
- Specificity:
- The structure of 5C6 against Sigma1 was solved crystallographically to 3.7 angstrom resolution (Dietrich et al, 2017). The 5C6 Fab regions each contact the upper part of the Sigma1 'head', which forms a beta-spiral repeat structure. The VH CDRs contact the antigen more extensively than the VL CDRs, resulting in a discontinuous binding interface. Reoviruses bind to cell-surface glycans as corecepto
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00926-10.0-BT
