Anti-HER2 [C7b]
Product Sizes
200 ug
AB00924-23-9-200UG
About this Product
- SKU:
- AB00924-23-9
- Additional Names:
- NEU; HER-2; HER-2/neu; ERBB2; CD340; MLN 19; NGL; TKR1; erb-b2 receptor tyrosine kinase 2; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2
- Application:
- Cell-based/Functional Assay, ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
- Buffer:
- PBS with 0.02% Proclin 300.
- translate.label.attr.clone:
- C7b
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS with 0.02% Proclin 300.
- Host:
- Rabbit
- Immunogen:
- A llama was immunized with the HER2-expressing ovarian cancer cell line SKOv3 and a sdAb phage display library was built and used to perform biopannings. The first round of selection was performed on a purified recombinant HER2 ectodomain fused to a human Fc portion (HER2-Fc) followed by a second round on an HER2-positive SKBr3 cell line. After selection, 188 clones were screened and 92 clones were found to be positive by ELISA on HER2 positive cells. Twenty clones were analyzed by sequencing, and all of them corresponded to a unique clone named C7b.
- Isotype:
- IgG
- Purification:
- Purified
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Specificity:
- C7b specifically recognises HER2 at a different epitope to that recognised by Herceptin. HER2 is a protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone.
- Storage Conditions:
- 2-8[o]C Aliquot.
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00924-23.9
