Anti-p21 [Y13-238]
Product Sizes
1 mg
AB00923-10-0-BT-1MG
About this Product
- SKU:
- AB00923-10-0-BT
- Additional Names:
- Ras; p21ras
- Application:
- Immunoprecipitation
- Buffer:
- PBS
- translate.label.attr.clone:
- Y13-238
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS only.
- Host:
- Human
- Immunogen:
- Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Human, Mouse
- Shipping Conditions:
- Blue Ice
- Specificity:
- The antibody reacts with v-H-ras (within amino acids 120-138) and rodent c-H-ras p21's but not v-K-ras or rodent c-K-ras p21's. This antibody also recognizes human c-H-ras and c-K-ras p21's, but not human c-N-ras.
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00923-10.0-BT
