Anti-Envelope protein DIII [E111]
Product Sizes
200 ug
AB00917-10-0-200UG
About this Product
- SKU:
- AB00917-10-0
- Additional Names:
- DENV1 DIII; Dengue virus 1 envelope protein DIII domain; DENV-1 envelope protein DIII domain; DENV-1 E protein DIII domain; DENV-1 E protein DIII
- Application:
- Block/Inhibit/Neutralise, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
- Buffer:
- PBS with 0.02% Proclin 300.
- translate.label.attr.clone:
- E111
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS with 0.02% Proclin 300.
- Host:
- Human
- Immunogen:
- Immunodeficient C57BL/6 background mice were infected with DENV-1 (genotype 2). In some cases, mice were boosted by injection of recombinantly produced DENV-1 E protein DIII. Splenocytes obtained from immunised mice were then fused with P3X63Ag8.53 myeloma cells to generate a hybridoma.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Virus
- Shipping Conditions:
- Blue Ice
- Specificity:
- DENV1-E111 was shown to bind the E protein DIII using yeast which specifically expressed each E protein domain (Shrestha et al, 2010). It was further shown that DENV1-E111 was specific for the type 1 DENV serotype, as well as genotype 2 of this serotype. Forward genetic screens could not identify DENV-1 DIII mutations which abrogated DENV1-E111 binding, however a crystal structure of a DENV1-E111
- Storage Conditions:
- 2-8[o]C Aliquot., -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00917-10.0
