Anti-Brg1 [4E5 ]
Product Sizes
200 ug
AB00905-10-0-200UG
About this Product
- SKU:
- AB00905-10-0
- Additional Names:
- SMARCA4; alpha-BRG1; BAF190; BAF190A; BRG1; MRD16; RTPS2; SNF2; SNF2L4; SNF2LB; SWI2; hSNF2b; CSS4; SWI/SNF related; matrix associated; actin dependent regulator of chromatin; subfamily a; member 4
- Application:
- Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
- Buffer:
- PBS with 0.02% Proclin 300.
- translate.label.attr.clone:
- 4E5
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS with 0.02% Proclin 300.
- Host:
- Human
- Immunogen:
- Anti-Brg1 rat monoclonal antibodies were generated based on the rat lymph node method. An 8-week-old female lzm rat was injected via the hind footpads with 200 mL of emulsion containing 100 mg of recombinant mouse Brg1 protein and Freund's complete adjuvant. After 3 weeks, the cells from the lymph nodes of a rat immunized with an antigen were fused with mouse myeloma Sp2/0-Ag14 cells at a ratio of 5:1 in a 50% polyethylene glycol solution. The resulting hybridoma cells were plated onto 96-wellplates and cultured in HAT selection medium. After 10 days post-fusion, the hybridoma supernatants were screened by means of an ELISA against GST-fused Brg1. Positive clones were subcloned and rescreened by ELISA and immunoblotting.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Human, Mouse
- Shipping Conditions:
- Blue Ice
- Specificity:
- mAb 4E5 specifically recognises alpha-Brg1
- Storage Conditions:
- 2-8[o]C Aliquot., -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00905-10.0
