Anti-S100A8 & S100A9 [5.5]
Product Sizes
1 mg
AB00815-1-1-BT-1MG
About this Product
- SKU:
- AB00815-1-1-BT
- Additional Names:
- MRP-8; MRP8; MRP-14; MRP14; CF antigen; CFAG; L1 molecule; p8,14; Calprotectin
- Application:
- ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
- Buffer:
- PBS
- translate.label.attr.clone:
- 5.5
- Clonality:
- Recombinant Monoclonal
- Formulation:
- PBS only.
- Host:
- Mouse
- Immunogen:
- BALB/c mice were immunized using cells from acute monocytic leukemia patients. The BALB/c spleen cell suspension was fused with Sp2/0-Ag14 cells, and mAb 5.5 was isolated from the hybridoma cells either by radioimmunoassay with 125-I-labelled F(ab')2 fragments of sheep anti-mouse Ig as second layer, or by indirect immunofluorescence using rhodamine-conjugated goat anti-mouse Ig as the second layer.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Specificity:
- mAb 5.5 specifically recognises complexed MRP-8/MRP-14 via the hydrophobic residues in helix IV of MRP-8 (S100A8) exposed during heterodimer formation. mAb recognition of MRP-8 & MRP-14 is calcium dependent. 5.5 epitope is most likely to be formed by linear sequence Phe26-His27-Ala28 in calcium-binding loop I of MRP-8, and by Phe68, Leu72, Ile76 and Lys77 that lie along one face of helix IV.
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00815-1.1-BT
